Diffusion pseudotime robustly reconstructs lineage branching
Autor/innen
- L. Haghverdi
- M. Büttner
- F.A. Wolf
- F. Buettner
- F.J. Theis
Journal
- Nature Methods
Quellenangabe
- Nat Methods 13 (10): 845-848
Zusammenfassung
The temporal order of differentiating cells is intrinsically encoded in their single-cell expression profiles. We describe an efficient way to robustly estimate this order according to diffusion pseudotime (DPT), which measures transitions between cells using diffusion-like random walks. Our DPT software implementations make it possible to reconstruct the developmental progression of cells and identify transient or metastable states, branching decisions and differentiation endpoints.