Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy
Authors
- M. Trumpp
- A. Oliveras
- H. Gonschior
- J. Ast
- D.J. Hodson
- P. Knaus
- M. Lehmann
- M. Birol
- J. Broichhagen
Journal
- Chemical Communications
Citation
- Chem Commun 58 (99): 13724-13727
Abstract
Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.